BIOMOLECULES IN GLYCOSMIS PENTAPHYLLA L. FRUITSEEDS MAY SERVE AS A HEPATOPROTECTIVE AGEN

Abstract

The primary purpose of this study was to determine the in vitro and Research Lab, GIET School of Pharmacy, NH-16, Chaitanya 
Knowledge City, Rajahmundry-533296. Research Lab, GIET School of Pharmacy, NH-16, Chaitanya Knowledge City, Rajahmundry533296. Research Lab, GIET School of Pharmacy, NH-16, Chaitanya Knowledge City, Rajahmundry-533296.in vivo Hepatoprotective 
activity of biomolecules that could be extracted from Glycosmis pentaphylla L fruit seeds. The DPPH test was used to determine the 
Hepatoprotective activity in vitro. The EEFSGP was measured to have an IC50 value of 212.96 g/ml (50% inhibition). For the in-vivo 
Hepatoprotective activity, albino rats were used. Rats treated with an ethanolic extract of Glycosmis pentaphylla fruit seeds showed a 
substantial (*P0.05) decrease in their CCl4-induced increased levels of SGOT, SGPT, ALP, and serum bilirubin (EEFSGP). The levels of 
SGOT, SGPT, ALP, and serum bilirubin were all reduced by 6.23% ns(non significantly), 28.96% ns(non significantly), 8.81% ns(non 
significantly), and 11.11 % ns(non significantly), respectively, after treatment with EEFSGP at a dose of 250 mg/kg b. wt. A higher dose of 
500 mg/kg b. wt. was A conventional dose of silymarin revealed inhibition by CCl4 of 55.09, 68.98, 57.46, and 35.04%, respectively. 
Based on these results, it can be concluded that the biochemical parameters of the ethanolic extract-treated group were considerably 
lower than those of the CCl4-treated group. The elevated levels of AST, ALT, ALP, and bilirubin in hepatotoxic rats were also 
dramatically decreased after treatment with the extract. Histopathological analysis demonstrated that the EEFSGP exhibited moderate to 
good hepatoprotective activity at both dosages (250 mg/kg b.w. and 500 mg/kg b.w. ), but at 500 mg/kg b.w. performed exceptional 
hepatoprotective efficacy against CCl4-induced injured hepatocytes.