A NOVEL VALIDATED RP-HPLC METHOD FOR ESTIMATION OFPROPIOMAZINE IN BULK AND PHARMACEUTICAL DOSAGE FORMS

Abstract

A simple, rapid, accurate, precise and reproducible RP-HPLC method was developed for the
estimation of Propiomazine in liquid dosage forms. The method was carried out using Inertsil
ODS 3V(150mm x 4.6 mm), 5µm column in an binary mode with mobile phase comprising
gradient mixture of pH 3.0 Potassium Di-Hydrogen phosphate and Acetonitrile. The flow rate
was 1.2 ml/min and detection was carried out at 260 nm using a UV detector. The retention time
for Propiomazine was found to be at 3.76 min and 9.74 min. The method for Propiomazine
showed linearity in the concentration range of 153.7- 461µg/ml (R2
=1.000) and for
Propiomazine showed linearity in the concentration range of 12.6-37.8µg/ml (r2
=0.9997). The
recovery studies for Propiomazine also carried out and %RSD for reproducibility was found to
be below 2%. The method was simple, sensitive and specific. Hence method can be used for the
quantification of Propiomazine in pharmaceutical dosage form.