Assessment of Baicalein-Loaded Hydrogel for Diabetic Wound Healing Management

Abstract

The goal of the current research was to thoroughly investigate the in-vivo wound healing impact of produced 
baicalein (BCA) loaded hydrogel and compare the results with the commercial formulation. In a prior 
investigation, prepared hydrogels were previously described and optimized. Glycol chitosan gellan gum polymers 
were used to create baicalein-loaded hydrogel (GG-GC-HGs). Rats with diabetes wound models (induced by 
streptozotocin) were used to assess the wound-healing potential of prepared hydrogels. Measurements of wound 
contraction and biochemical analyses (Hydroxyproline, protein content, and antioxidant test) in the treated wound 
tissue were used to assess the impact of wound healing. Hematological analysis of the tissue from the wound was 
done. The study's findings demonstrated that after 10 days of therapy, the percentage of wound contraction in the 
animal group treated with baicalein loaded GG-GC-HGs decreased significantly (p < 0.05), and on day 18, the 
wounds fully healed. Treatment of baicalein-loaded GG-GC-HGs resulted in a considerable increase in 
hydroxyproline and protein content; the findings were equivalent to those of the animal reference group 
(Hydroheal Gel). Following treatment with BCA-loaded GG-GC-HGs, antioxidant status was recovered. These 
findings were corroborated by histological examination of the wound tissues. In conclusion, baicalein-loaded 
hydrogel significantly improved diabetic wound healing by promoting fibroblast proliferation, enhancing 
epithelialization, and lowering oxidative stress