Creating and testing an RP-HPLC technique to measure curcumin and metronidazole in a mixed dose form at the same time

Abstract

Purpose and Background: This work set out to create and test a straightforward RP-HPLC technique for the 
simultaneous measurement of curcumin and metronidazole in bulk, as well as their combination dose form. The 
compounds in question are natural.
Methods: A model combination system was created using an in situ gel formulation that included metronidazole and 
curcumin. Using UV-detection at 254 nm, the chromatographic separation was achieved isocratically on an Eclipse 
XDB-C18 column with dimensions 150 mm x 4.6 mm and a particle size of 5 μm. The mobile phase that was finetuned included a 50:50 (v/v) combination of Phosphate Buffer pH4.5 and Acetonitrile. The flow rate was adjusted to 
1.0 mL/min, and the injection volume was 10 μL. The approach was utilized for quality control tests of their 
combination medication product and was verified in conformity with International Council for Harmonization (ICH) 
standards.
The findings showed that metronidazole had a retention duration of 1.40 minutes and curcumin of 8.60 minutes. The 
linear responses were seen for curcumin and metronidazole, respectively, throughout the concentration ranges of 3.0-
80 and 4.8-128 μg/mL, with limits of detection (LOD) values of 0.62 and 1.03 μg/mL and limits of quantification 
(LOQ) values of 1.88 and 3.13 εg/mL. No formulation components interfered with the identification of the two active 
compounds, and the precision findings were within acceptable limits (RSD<2%).
Curcumin and metronidazole in situ gel formulation total contents were determined using the suggested verified RPHPLC technique. Routine quality control for their combined pharmaceutical use was shown to be possible by the 
validation findings, which also demonstrated that the suggested technique was simple, specific, and exact.
Developing an HPLC approach for the simultaneous measurement of curcumin and metronidazole