Amaranthus tricolor Linn. Leaf Extracts' In-vitro Anthelmintic Potential

Abstract

Using acetone, ethyl acetate, and ethanol as solvents, the goal of the current research was to examine the 
anthelmintic potential of the Amaranthus tricolor Linn plant, which belongs to the Amaranthaceae family. The 
phytochemical components of the extracts were tested, and their vermicidal efficacy against adult Eisenia fetida 
earthworms was assessed. Comparatively speaking, phytochemicals were present in all of the extracts. The 
majority of the phytochemicals were present in the acetone extract, but the ethanol extract had less of them. In 
the bioassay, different concentrations (10–30 mg/mL) of each extract were examined, and the earthworms' 
paralysis and eventual death were tracked. Normal saline was used as the control group and albendazole as 
the reference standard. Every extract showed greater potency than the reference medication and dosedependent anthelmintic action in both the measures (paralysis and death). The most promising result was the 
acetone extract (30 mg/mL), which paralyzed worms in 5 minutes and killed them in 13 minutes. The result 
implies that the A. tricolor Linn plant's acetone extract could be helpful as an anthelmintic. The current 
research provides scientific proof for the traditional use of this leafy vegetable as a vermicide and indicates 
that the leaves of the A. tricolor Linn plant are a good source of active chemicals with anthelmintic action. 
According to the early phytochemical study, the remarkable vermicidal activity of acetone extract may be 
attributed to the substantial presence of glycosidic and phenolic compounds in it.