INVESTIGATING THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI IN DAIRY PRODUCTS

Abstract

Background and Objective: Raw milk 
has the potential to transmit antibioticresistant bacteria and be a vehicle for the 
development of food-borne illnesses. 
Both Escherichia coli and 
Staphylococcus aureus are dangerous 
bacteria that can assault host cells and 
carry genes resistant to antibiotics. In 
East Java, Indonesia, this study assessed 
the prevalence and antibiotic resistance 
of S. aureus and E. coli isolated from 
raw milk.
Supplies and Procedures: In East Java, 
two hundred and fifty raw milk samples 
were taken from five dairy farms. The 
Kirby-Bauer disc diffusion method was 
used to test for antibiotic susceptibility 
after S. aureus and E. coli were isolated 
using their respective selective mediums. 
The oxacillin resistance screen agar test 
was used to validate the presence of 
methicillin-resistant S. aureus (MRSA), 
and the double-disk synergy test was 
used to identify E. coli that produced 
extended-spectrum beta-lactamase 
(ESBL). The polymerase chain reaction 
method was used to screen for the 
presence of the mecA and blaTEM 
genes.
The findings showed that 138 (55.2%) 
people had S. aureus and 176 (70.4%) 
people had E. coli. Out of the 138 
isolates of S. aureus, 27 (19.6%) were 
MRSA, and 3 (1.7%) of the 176 isolates 
of E. coli were ESBL producers.
Two (7.4%) MRSA were found to have 
the mecA gene, while all three (100%) 
of the recovered ESBL-producing E. 
Coli were found to have blaTEM genes.
In summary, the existence of MRSA and 
ESBL-producing E. coli in raw milk 
poses a significant risk to public health, 
and it is imperative to increase public 
awareness of the dangers associated with 
these pathogenic organisms.