Chromolaena odorata methanol leaf extract as a wound healing agent and antioxidant: a rat model study

Abstract

People have been utilizing plants and other natural things as remedies for a long time. At the moment, conventional medicine is in vogue. In terms of disease prevention and treatment, the many phytochemicals found in plants may work in tandem via a variety of pathways. This research examined the effects of Chromolaena odorata methanol leaf extract on wound healing and antioxidant activity in adult male rats. The leaf extract was evaluated for its phytochemical composition, acute toxicity, anti-oxidative capabilities, and wound healing capabilities. Chromolaena odorata methanol leaf extract was used in an anti-oxidative investigation to see how it affected malondialdehyde (MDA) and catalase. The rats' backs were cut in an excision wound healing experiment, and three different dosages of Chromolaena odorata methanol leaf extract embedded in petroleum jelly were applied to the wounds. The positive control consisted of cikatrin powder, whereas the negative control was petroleum jelly. Among the phytochemicals found were steroids, alkaloids, flavonoids, proteins, and tannins. A half-life of 5,000 mg/kg of body weight was recorded. The anti-oxidative experiment demonstrated that the leaf extract effectively inhibited lipid peroxidation. In contrast to the 14.16 ± 0.59 x 10-5 μmol/ml measured with distilled water, the MDA was lowered to 5.63 ± 0.33 x 10-5 μmol/ml by the 500 mg/kg body weight extract. After 12 weeks of therapy, the wound healing percentage for the 5% and 10% extracts was 95.52% and 97.14% respectively, which was higher than cikatrin's 87.95%. Chromolaena odorata methanol leaf extract outperformed cikatrin in terms of wound healing potency and had a favorable safety profile.