CHROMATOGRAPHIC METHOD DEVELOPMENT AND VALIDATION FOR NEPAFENAC AND DEXAMETHASONE IN COMBINATION

Abstract

As per the overlain spectrum the wavelength selected for estimation of Nepafenac was 378 nm, 
where Dexamethasone has no significant absorbance and for Dexamethasone it was 237 nm, 
where absorbance of Dexamethasone is corrected. correlation coefficient of 0.999 and 0.999 
for Nepafenac and Dexamethasone respectively. The suggested approach was assessed as wellby the Assay of Synthetic mixture containing Nepafenac and Dexamethasone. The 
% assay was observed 99.94 % for Nepafenac and 100.06% for Dexamethasone in mixture 
respectively. The % recovery for Nepafenac was got to be 99.39 to 99.71% and for 
Dexamethasone was observed 99.65 to 99.97% LOD and LOQ was got 0.014µg/ml and 0.043
µg /ml for Nepafenac, respectively and 0.012µg/ml and 0.036µg/ml for Dexamethasone, 
respectively.